FIG. 3.

Time course of thaumatin production by selected transformants with the different promoters in flask cultures with the nitrogen and carbon sources shown in Table 1. (A) Total production of thaumatin. (B) Specific production of thaumatin. ⧫, TGDTh-4; ●, TB2b1-44; ✶, TGP-3; ■, TCTh-21; ⊙, TB2b1-44-GD5. The vertical thin lines indicate the standard deviation of the four determinations at each sample point. (C) Immunoblot detection of thaumatin produced at different times during fermentation by strain TB2b1-44-GD5 (A). The first lane contains a control of purified 22-kDa thaumatin from T. daniellii (100 ng).