FIG. 4.

Southern blot hybridizations of A. awamori lpr66 transformants and an untransformed control strain (lpr66) with a ³²P-labeled 332-bp NcoI fragment internal to tha and a 694-bp PvuII probe from the gdhA gene. Genomic DNA was digested with the restriction enzymes NcoI and BamHI, and the fragments were separated on a 0.8% agarose gel and transferred to a nylon filter. Numbers on the left indicate the molecular size (in kilobases) of λ-HindIII standards. The copy number of the thaumatin gene in each transformant (relative to one copy per genome of gdhA) is indicated on the bottom of the figure.