FIG. 6.

(A) Elution profile of recombinant thaumatin from a carboxymethyl-Sepharose column with an NaCl gradient (0 to 400 mM). Peaks A, B, and C represent the three purified forms of thaumatin. (B) SDS-PAGE (left) and immunoblotting (right) analysis of purified recombinant thaumatin. Lanes: 1, molecular mass markers (Pharmacia low-molecular-weight calibration kit); 2, commercial thaumatin from Thaumatococcus (Sigma); 3, fraction 29 from carboxymethyl-Sepharose elution (peak A); 4 and 5, fractions 33 and 34, respectively (peak B); 6, fraction 39 (peak C).