Fig. 5.

Comparing germacrene D synthases for high germacrene D production in S. cerevisiae. A Schematic diagram of comparing germacrene D production in S. cerevisiae. Transformation, flask fermentation and quantitative analysis were performed as described in “Methods” (B) Strategy for the construction of SC1-SC21 via homologous recombination. JCR27, the starting strain; SC1-SC21, yeast strains expressing different germacrene D synthases; ROX1, the insertion site for exogenous fragments; LB (ROX1), the upstream sequence of rox1; RB (ROX1), the downstream sequence of ROX1; tCYC1, the terminator of CYC1; X-Sc, the germacrene D gene optimized for S. cerevisiae; pGAL1pGAL10, the promoter of GAL1 and GAL10. B The production of different germacrene D producing strains. Germacrene D levels were determined after 72 h fermentation in YPDH medium as described in the “Methods”. Error bars represent standard deviations from three independent experiments. ND, not detected. SSLH2 et al., the names of germacrene D synthases from different organisms detailed in the text