MboI-digested P. aeruginosa flagellin gene PCR products. The gel shows all of the patterns (patterns A through H) obtained with restriction enzyme MboI. Restriction pattern E is shown twice; it was derived once from a clinical isolate and once from a mushroom unit isolate. Lanes M contained a PCR marker (fragment sizes, 50, 150, 300, 500, 750, 1,000, 1,500, and 2,000 bp).