. Author manuscript; available in PMC: 2022 May 18.

Published in final edited form as: Biochem Pharmacol. 2020 Oct 17;183:114291. doi: 10.1016/j.bcp.2020.114291

Table 1.

Fourth generation MAPS mutants based on PnCS1.

Peptide	Sequence	Peptide	Sequence

PnCS1	CRRWARWNRC^*	Glu mutants
Ala mutants		PnCS1[R2E]	CERWARWNRC^*
PnCS1[R2A]	CARWARWNRC^*	PnCS1[R3E]	CREWARWNRC^*
PnCS1[R3A]	CRAWARWNRC^*	PnCS1[W4E]	CRREARWNRC^*
PnCS1[W4A]	CRRAARWNRC^*	PnCS1[A5E]	CRRWERWNRC^*
PnCS1[R6A]	CRRWAAWNRC^*	PnCS1[R6E]	CRRWAEWNRC^*
PnCS1[W7A]	CRRWARANRC^*	PnCS1[W7E]	CRRWARENRC^*
PnCS1[N8A]	CRRWARWARC^*	PnCS1[N8E]	CRRWARWERC^*
PnCS1[R9A]	CRRWARWNAC^*	PnCS1[R9K]	CRRWARWNEC^*
Lys mutants		Tyr mutants
PnCS1[R2K]	CKRWARWNRC^*	PnCS1[W4Y]	CRRYARWNRC^*
PnCS1[R3K]	CRKWARWNRC^*	PnCS1[W7Y]	CRRWARYNRC^*
PnCS1[W4K]	CRRKARWNRC^*	Acid C-terminal
PnCS1[A5K]	CRRWKRWNRC^*	PnCS1DeAm	CRRWARWNRC
PnCS1[R6K]	CRRWAKWNRC^*	Acetylation of N-terminal
PnCS1[W7K]	CRRWARKNRC^*	PnCS1AcAm	Ac-CRRWARWNRC^*
PnCS1[N8K]	CRRWARWKRC^*	Acetylation of N-terminal and acid C-terminal
PnCS1[R9K]	CRRWARWNKC^*	PnCS1AcDeAM	Ac-CRRWARWNRC

All peptides were N- to C-terminal cyclised via a disulfide bond. Mutations are underlined in bold; Ala-mutants in red, Lys-mutants in blue, Glu-mutants in green, Tyr-mutations in brown and acetylation in black.

- amidated C-terminal, Ac - acetylated N-terminal.