Figure 1.

Inhibiting mitochondrial pyruvate import during CD8⁺ T cell activation favors memory differentiation

(A) Listeria experiment.

(B) Number of transferred cells in the blood.

(C–E) Percentage of SLECs (C) and MPECs (D) at 1 week and T_CM cells (E) at 4 weeks post-infection, out of transferred cells in the blood.

n = 11–12 mice/group in (B)–(D) or 7–8 mice/group in (E); pooled data from 2 independent experiments.

(F and G) Percentage of T_CM cells (F) and number of transferred cells (G) in the spleen 60 days post-infection (F) (n = 7 mice [WT] versus 5 mice [MPC1 KO]; pooled data from 2 independent experiments).

(H) At 60 days post-infection, transferred cells were FACS-sorted from spleens and retransferred in new host, followed by Listeria infection. Expansion of the retransferred cells was measured in the blood at day 6 post-infection (n = 12–14 mice/group; pooled data from 2 independent experiments).

(I) Experimental scheme (MPCi = 20 μM UK5099).

(J) Number of transferred cells in the blood.

(K–N) Percentage of SLECs (K), MPECs (L), and TCF1-positive cells (M) at 1 week and T_CM cells (N) at 8 weeks post-infection, out of transferred cells in the blood (n = 10 mice/group; pooled data from 2 different experiments).

Data are represented as mean ± SEM. Statistics are based on unpaired, two-tailed Student’s t test (C–H) or two-way ANOVA (K–N), ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001, and ns (p > 0.05).