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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Nature. 2021 Dec 22;601(7893):428–433. doi: 10.1038/s41586-021-04244-1

Extended Data Fig. 10 |. Loss of pathway intrinsic ETC proteins promotes resistance to valine restriction.

Extended Data Fig. 10 |

a, Validation of NDUFB4 as a positively selected gene in low valine media relative to standard valine media using a competition-based proliferation assay in Cas9 expressing cell lines CUTLL1 and DND41. Plotted are GFP percentages measured during 20 days in culture and normalized to day 4. Negative control sgRosa and two independent sgRNAs targeting NDUFB4 are shown in the graphs (mean ± SD of n=3 independent replicates). b, Schematic depicting how upregulated valine tRNA biogenesis regulates mitochondrial bio-energetics in T-ALL (created with Biorender.com).