Fig. 2 |. Dietary valine restriction reduces leukaemic burden and increases survival.

a, Representative FACS plots of GFP⁺ frequency in peripheral blood of mice transplanted with NOTCH-ΔE–GFP⁺ cells and fed either a control diet (+valine) or a valine-deficient diet (−valine). b, Percentage of GFP⁺ cells in peripheral blood of transplanted mice fed either a control diet (n = 10) or a valine-deficient diet (n = 10) followed by gradual valine reintroduction (mean ± s.d.; two-sided unpaired t-test). c, Kaplan–Meier survival graph of mice represented in b (log-rank test; two-sided). d, Leukaemic burden in peripheral blood in mice transplanted with patient-derived xenograft model 1 of T-ALL fed either a control diet or a valine-deficient diet (n = 5; mean ± s.d.; two-sided unpaired t-test) along with representative spleen images. e, Kaplan–Meier survival graph of mice transplanted with patient-derived xenograft 2 and fed a control diet or a valine-deficient diet (n = 5 each condition) (log-rank test; two-sided). Death owing to leukaemia or toxicity of valine deprivation is highlighted. f, Absolute leukaemic burden in peripheral blood from mice fed different dietary valine diets: 8 g kg⁻¹ valine (n = 4), 0 g kg⁻¹ valine (n = 5), 0 g kg⁻¹ valine substituted with either 0.8 g l⁻¹ valine (n = 5) or 0.4 g l⁻¹ valine (n = 5) (mean ± s.d.; two-sided unpaired t-test). g, Kaplan–Meier survival graph of mice fed different levels of dietary valine (8 g kg⁻¹ (n = 10); 0 g kg⁻¹ (n = 10); 0 g kg⁻¹ substituted with 0.8 g l⁻¹ valine (n = 7) or 0.4 g l⁻¹ valine (n = 9) (log-rank test; two-sided). NS, not significant. h, Representative images of the spleen from mice fed different levels of dietary valine.