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Ferroptosis induction mediated the underlying effects of RGP treatment. A549 and MDA-MB-231 cells were pre-treated with 200 μg/mL of RGP, and then ferrostatin-1 (2 μM) or erastin (35 μM) was added to cells. (A, B) Cell viability assay, (C, D) LDH release assay and (E, F) C11-BODIPY assay were performed. (G, H) GPX4 expression level was determined. *p < 0.05, **p < 0.01 and ***p < 0.001.
