TABLE 2.
Rates of Sr2+ accumulation by S. cerevisiae enriched with a range of fatty acidsa
| Fatty acid supplement (isomer) | Sr2+ accumulation at fatty acid concn (mM) suppliedb
|
|||
|---|---|---|---|---|
| 0 | 0.025 | 0.1 | 1.0 | |
| 16:1 (n-7) | 0.54 ± 0.02 | 0.50 ± 0.01 | 0.49 ± 0.03 | 0.42 ± 0.05 |
| 18:2 (n-6) | 0.54 ± 0.02 | 0.68 ± 0.04c | 0.71 ± 0.03c | 0.86 ± 0.07c |
| 18:3 (n-3) | 0.54 ± 0.02 | 0.56 ± 0.03 | 0.52 ± 0.01 | 0.51 ± 0.03 |
| 18:3 (n-6) | 0.54 ± 0.02 | 0.54 ± 0.01 | 0.48 ± 0.03 | 0.50 ± 0.05 |
| 20:2 (n-6) | 0.54 ± 0.02 | 0.46 ± 0.03 | 0.32 ± 0.06c | 0.28 ± 0.07c |
| 20:2 (n-9) | 0.54 ± 0.02 | 0.44 ± 0.01c | NDd | ND |
Cells previously grown in medium supplemented with the appropriate fatty acid were incubated in MES buffer with 1% (wt/vol) glucose and exposed to 100 μM Sr(NO3)2.
The Sr2+ accumulation rate is given in pmoles (106 cells)−1 minute−1, determined during the linear accumulation phase (see text). Values are means ± standard errors of the means, based on three determinations. Typical results from one of at least two independent experiments are shown.
Result that is significantly different (P < 5.0%) from the control rate (0.54 ± 0.02).
ND, not determined.