Fig. 4. Real-time in vivo visualization of CB2R in zebrafish embryos. (A) 8-SiR (red) injected in 3dpf Tg(fli1:EGFP) zebrafish larvae, which expresses EGFP fluorescent protein in the blood vasculature (cyan). 8-SiR was found freely circulating in the blood vasculature after injection, with increased intensity in the CHT region (white triangles). (B) 8-SiR injected in 3dpf Tg(mpeg1.1:mCherry-F) zebrafish larvae, which expresses mCherry-F fluorescent protein in macrophages (cyan), demonstrating its colocalization. (C) 7-AF488 (red) injected in 3dpf Tg(kdrl:mCherry) zebrafish larvae, which expresses mCherry fluorescent protein in the blood vasculature (cyan). See also ESI Videos S8 and S9.†.
