Figure 3.

AT2 cell binucleation is not universally associated with Krt8-high expression phenotype. (A) Experimental design as in Figure 1. Confocal image of lung section from bleomycin-treated mice; same image as Figure 2A, now including Krt8 immunofluorescence channel (cyan). Asterisk denotes binucleated AT2 cell with normal levels of Krt8 protein. Native GFP fluorescence from AT2-lineage label and immunofluorescence staining of AT1 cells by Pdpn (magenta). DNA labeling by Hoechst (gray). (B) Confocal image of binucleated Krt8-high cell (asterisk) lacking AT2 lineage label. Scale bars, 50 μm and 25 μm (inset). (C) Confocal image of lineage-labeled binucleated AT2 cell adjacent to lineage-negative mononuclear Krt8-high cell (z-stack not shown). Scale bar, 25 μm. Krt8 = keratin 8.