Table 2.

Statistics of label databases reviewed in this paper

Database	Methods	Description	Species and No. of SL pairs	Website	Latest update
SynLethDB V2 [34]	DAISY, text mining, large-scale screening techniques	Comprehensive database for SL	H. sapiens: 35943 S. cerevisiae: 14000 D. melanogaster: 439 M. musculus: 381 C. elegans: 105	http://synlethdb.sist.shanghaitech.edu.cn/v2/#/	2020
BioGRID V 4.4.201 [35–37]	Experiments and literature mining	Genetic interactions from all major model organisms and humans	Major model organisms and humans	http://www.thebiogrid.org	1 September 2021
Syn-lethality [38]	Manually curated SL pairs for human cancer from the literatures (113) SL pairs for human cancer inferred from yeast (1114)	Integrates experimentally discovered and verified human SL gene pairs into a network	H. sapiens: 1227	http://www.ntu.edu.sg/home/zhengjie/software/Syn-Lethality/ (NTU staff’s personal web pages)
GenomeRNAi [39]	RNAi	Genetic interactions detected by GenomeRNAi	H. sapiens Drosophila	http://www.genomernai.org/	27 November 2017
DAISY [13]	Computational prediction	Statistically inferring SL pairs	H. sapiens: 2816	http://www.cs.tau.ac.il/∼livnatje/SL_network.zip
The Cellmap [40]	Yeast screening	Database of genetic interaction for S. cerevisiae	S. cerevisiae: ~10 000 (GI score < −0.35)	http://thecellmap.org	May 2016
Laufer et al. study [42]	RNAi	Combinatorial RNAi and high-throughput imaging	Human cell lines: HCT116 HeLa	http://www.bioconductor.org/packages/2.12/data/experiment/html/HD2013SGI.html
Vizeacoumar et al. study [43]		A negative genetic interaction map in isogenic cancer cell lines	6 isogenic cancer cell lines (KRAS, PTTG1, PTEN, MUS81, BLM)	Support Information http://kimLab1.ccbr.utoronto.ca/projects/cancer_essential/ or http://moffatlab.ccbr.utoronto.ca/resources.php
Shen et al. study [44]	CRISPR screening	Combinatorial CRISPR screening	Human cell lines HeLa: 52 A549: 57293 T: 59	293 T - http://www.ndexbio.org/#/newNetwork/199f9bb1-c3eb-11e6-8e29-06603eb7f303 A549 - http://www.ndexbio.org/#/newNetwork/ec8bdae3-c3c9-11e6-8e29-06603eb7f303; HeLa - http://www.ndexbio.org/#/newNetwork/e50ee3c2-c3d4-11e6-8e29-06603eb7f303.
GImap [12]		Combinatorial CRISPR screening	Human cell lines Jurkat: 454 K562:1678	https://data.mendeley.com/datasets/rdzk59n6j4/1	22 July 2018
Najm et al. study [45]		Combinatorial CRISPR screening	Human cell lines A375, HT29, OVCAR8, 786O, A549, Meljuso
Zhao et al. study [46]		Metabolic gene networks through combinatorial CRISPR screening	Human cell lines A549 HeLa	Support information
GEMINI [47]	Computational prediction	A variational Bayesian approach to identify genetic interactions from combinatorial CRISPR screening	Sensitive lethal interactions and sensitive recovery interactions for four combinatorial CRISPR studies	Support information
Wan et al. study [41]		Application of GEMINI to identify genetic interactions	Human cell lines A549: 126 A375: 18 HT29: 18	https://github.com/FangpingWan/EXP2SL/tree/master/GEMINI
Slorth [25]		Predict SL pairs in a RF classifier	H. sapiens: 518636 S. cerevisiae: 372560 D. melanogaster: 93002 S. pombe: 52594 C. elegans: 56908	http://slorth.biochem.sussex.ac.uk	Jun, 2019
CGIdb [48]		Identify potential SL pairs for specific cancer types from TCGA and functional screen data	H. sapiens: 10 637	http://www.medsysbio.org/CGIdb	2019
Srivas et al. study [49]	Drug screening	Evaluate thousands of TSG-drug combinations	Yeast: 1420 HeLa: 127	Support information	2016

Note: BioGRID, Biological General Repository for Interaction Datasets; DAISY, Data mining SL identification pipeline; TCGA, The Cancer Genome Atlas; TSG, tumor suppressor genes; H. sapiens, Homo sapiens; S. cerevisiae, Saccharomyces cerevisiae; D. melanogaster, Drosophila melanogaster; M. musculus, Mus musculus; C. elegans, Caenorhabditis elegans; S. pombe, Schizosaccharomyces pombe.