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VapC2 and VapC21 inhibit protein synthesis. Newly synthesized proteins were labeled with the methionine mimetic AHA in induced and uninduced samples 1, 3, or 5 days post-induction of either VapC2 (A) or VapC21 (B) and visualized upon alkyne-TAMRA conjugation. Each lane was normalized to 10 μg of total protein.
