. 2022 Apr 12;66(5):e01725-21. doi: 10.1128/aac.01725-21

TABLE 4.

Primers used for amplification and sequencing of HS1 and HS2 regions of FKS genes

Species	Region	Primer	Sequence 5′ 3′	Ref
Candida albicans	HS1	GSC1f	GAAATCGGCATATGCTGTGTC	36
		GSC1r	AATGAACGACCAATGGAGAAG	36
	HS2	CAS2f	ACCACCAAGATTGGTGCTG	17
		CAS2r	TATCTAGCACCACCAACGG	17
Pichia kudriavzevii	HS1	CKS1f	ACTGCATCGTTTGCTCCTCT	17
		CKS1r	GAACATGATCAATTGCCAAC
	HS2	CKS2f	CCGGTATGGGAGAACAAATG
		CKS2r	CACCACCAATGGAAACATCA
Candida tropicalis	HS1	CTS1-1f	ATGGTTCAGTATAGGTGGATG	17
		CTS1-1r	AAGGAACGACCAATGGAGAAG
	HS2	CTS1-2f	ACTACCAAGATTGGTGCTG
		CTS1-2r	TATCTAGCACCACCAACAG
Candida glabrata	FKS2-HS1	FKS2F	GGCCACTGTTTTATTCTTCTCG	35
		FKS2R	GTAAATGTTCTCTGTACATGGA	35
	FKS2-HS2	CG2f	ACAACTAAGATTGGTGCAG	54
		CG2r	TAACGAGCACCACCCACA	54
	FKS1-HS1	FKS1f	GTTGCAGTCGCTACATTGCTA	35
		FKS1r	TAGCGTTCCAGACTTGGGAA	35
	FKS1-HS2	FKS1HS2f	GGTATTTCAAAGGCTCAAAAGGG	39
		FKS1HS2r	ATGGAGAGAACAGCAGGGCG	39
	FKS3-HS1	FKS3f	TGGAGCCCAGCACTTAACAA	35
		FKS3r	GTCCATCTCGGATGTTGCTA	35
	FKS3-HS2	CG3-HS2f	TTATGCAGAGGAACCTGCTC	54
		CG3-HS2r	GTGCCATCGACAGTAAGTGA	54
Kluyveromyces marxianus	HS1	CKHS1f	GGTGGTTTATTCACTTCCTACA	42
	HS1	CKHS1r	GCGTAGCCAAAGATTGAGCA
	HS2	CKHS2f	AAGATTGGTGCYGGTATGGG
	HS2	CKHS2r	RGTDGCAAAACCTCTAGCAGT