Figure 3.

Effect of ethanol consumption on macrophage and neutrophil numbers in the lung following infection. (A) Flow cytometry gating strategy to identify neutrophils, alveolar macrophages, and infiltrating macrophages in lung homogenates. (B) Average number of pulmonary cell subsets in lung homogenate at 24 hours post-infection. (C) RNA from lung homogenates was isolated at 24 hours post-infection and cDNA was analyzed by quantitative PCR for expression of Ly6g; target gene expression is normalized to gapdh and presented as fold change to vehicle-treated uninfected mice. Data are presented as mean ± SEM. n = 3-6 mice per group per experiment and data are combined from 2 individual experiments. *p < 0.05 compared to all other groups by one-way ANOVA.