Figure 6.

Effect of ethanol exposure on neutrophil and S. pneumoniae localization and bacterial phagocytosis after infection. (A) IHC staining was performed onformalin-fixed lung sections with antibodies against Ly6G for neutrophils (brown) and S. pneumoniae (pink) and counterstained with hematoxylin. Representative images are at 100x magnification for the top panel (scale bar = 200μm) and 400x magnification for the bottom panel (scale bar = 50 μm). (B) Percent phagocytosis of S. pneumoniae as measured by the ratio of cells with internalized bacteria to total nucleated cells. Black arrows denote cells with internalized S. pneumoniae. Data are presented as mean ± SEM. n = 3-4 mice per group per experiment and are representative of 2 individual experiments. *p < 0.05 by unpaired t test.