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. 1999 Mar;65(3):1228–1235. doi: 10.1128/aem.65.3.1228-1235.1999

FIG. 2.

FIG. 2

Southern analysis of six independent FS mutants of S. meliloti RMB7201. Panel A shows FS1 (lanes 1 and 4), FS7 (lanes 2 and 5), and FS44 (lanes 3 and 6) probed with the 3.2-kb HindIII fragment from wild-type Tn5. Panel B shows FS17 (lanes 1 and 4), FS27 (lanes 2 and 5), and FS28 (lanes 3 and 6) probed with the 5.0-kb EcoRI-BamHI fragment from mini-Tn5-lacZ1. RMB7201 DNA has no detectable sequence homology to wild-type Tn5 but shows a faint band when probed with the mini-Tn5 sequence. The values between the lanes are approximate molecular sizes in kilobases.