TABLE 3.
Chemotactic responsiveness of wild-type S. meliloti RMB7201 and mutant FS1
| Attractant (conc) | Avg chemotaxis ratioa ± SD
|
|
|---|---|---|
| RMB7201 | FS1 | |
| Glutamine (10 mM) | 37 ± 5.8 | 43 ± 5.2 |
| Glucose (30 mM) | 1.6 ± 0.28 | 1.8 ± 0.2 |
| Succinate (30 mM) | 3.2 ± 0.8 | 3.7 ± 0.5 |
| Alfalfa seed exudate (1/4) | 93 ± 21 | 125 ± 17 |
| Alfalfa seed exudate (1/16) | 53 ± 16 | 66 ± 4.7 |
| Alfalfa seed exudate (1/64) | 18 ± 5.8 | 22 ± 1.9 |
a
The chemotaxis ratio is the average number of cells entering attractant-filled capillaries divided by the average number of cells (ca. 3,000/30 min) entering buffer-filled control capillaries. Ratios are based on average numbers of CFU from four replicate capillary tubes and are from a single experiment that was repeated with similar results. Differences in the chemotaxis ratio between the wild type and FS1 were not significant at the 95% confidence level by t-test analysis.