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. 1999 Mar;65(3):1228–1235. doi: 10.1128/aem.65.3.1228-1235.1999

TABLE 6.

Motility and flagellation of FS mutants

Strain Time after resuspension in CBa
0 h
4 h
% Motileb % Flagellatedc No. of Flagella/celld Avg flageller lengthe (μm) % Motileb % Flagellatedc
RMB7201 42 47 1.9 2.4 33 44
FS1 55 57 2.4 3.8 43 55
FS7 53 46  NDf ND 35 44
FS44 53 51 ND ND 35 46
a

Cells from log-phase 1/10 TY cultures were washed and gently resuspended in CB. 

b

Motile-cell percentages are averages of counts from several fields and two independent cultures from a representative experiment. Averages from replicate aliquots normally varied by ±5%. 

c

Flagellated-cell percentages are averages of counts from about 60 to 100 cells on two separate grids from the same two independent cultures used for motile-cell percentage assays. Averages from replicate grids normally varied by less than 5%. 

d

Average number of flagella per flagellated cell, determined from electron micrographs of at least 100 cells per strain on replicate grids. Values were reproducible within ± 10% between grids. 

e

Average length of flagella per flagellated cell, determined by planimetry from micrographs of at least 50 cells per strain on replicate grids. 

f

ND, not determined.