TABLE 6.
Strain | Time after resuspension in CBa
|
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---|---|---|---|---|---|---|
0 h
|
4 h
|
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% Motileb | % Flagellatedc | No. of Flagella/celld | Avg flageller lengthe (μm) | % Motileb | % Flagellatedc | |
RMB7201 | 42 | 47 | 1.9 | 2.4 | 33 | 44 |
FS1 | 55 | 57 | 2.4 | 3.8 | 43 | 55 |
FS7 | 53 | 46 | NDf | ND | 35 | 44 |
FS44 | 53 | 51 | ND | ND | 35 | 46 |
Cells from log-phase 1/10 TY cultures were washed and gently resuspended in CB.
Motile-cell percentages are averages of counts from several fields and two independent cultures from a representative experiment. Averages from replicate aliquots normally varied by ±5%.
Flagellated-cell percentages are averages of counts from about 60 to 100 cells on two separate grids from the same two independent cultures used for motile-cell percentage assays. Averages from replicate grids normally varied by less than 5%.
Average number of flagella per flagellated cell, determined from electron micrographs of at least 100 cells per strain on replicate grids. Values were reproducible within ± 10% between grids.
Average length of flagella per flagellated cell, determined by planimetry from micrographs of at least 50 cells per strain on replicate grids.
ND, not determined.