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. 2022 Jun 1;13(3):943–959. doi: 10.14336/AD.2021.1029

Figure 6.

Figure 6.

C3d+/GFAP+ astrocyte derived medium reduced tight junction protein expression in vitro. (A) Immunofluorescence images showed resting astrocytes were converted to C3d+/GFAP+ cells (C3d in red color; GFAP in green color; DAPI in blue color) after treated with IL-1α, TNFα and C1q. Scale bar=25 μm. (B) Bar graph showed the mRNA levels of C3d+/GFAP+ cells related genes H2-T23, Serping1, H2D1 and Ligp1 expression after IL-1α, TNFα and C1q treatment. Data are mean±SEM. n=3 per group. *p<0.05, ***p<0.001. (C) Photomicrographs showed tight junction proteins (claudin-5 and ZO-1 in red color) expressed in CD31+ cells (green) that were treated by 1) medium derived from inactivated astrocytes (A0-CM), 2) medium derived from IL-1α, TNFα and C1q treated astrocytes (A1-CM) and 3) medium derived from astrocytes that were treated with LPS-stimulated microglia (LPS-MCM-AS-CM). Scale bar=25 μm. (D) Western blotting analysis of claudin-5 and ZO-1 in the A0-CM group, A1-CM group and LPS-MCM-AS-CM group. n=3 per group. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.