Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| antibody | anti-GFP (rabbit polyclonal) |
Invitrogen | A11122 | (1:1000) |
| antibody | anti-FLAG M2 (mouse monoclonal) | Sigma | F1804 | (1:500) |
| antibody | IRDye 800CW Donkey anti-rabbit IgG Secondary Antibody (clonality unspecified) | LI-COR | 926–32213 | (1:10,000) |
| antibody | IRDye 800CW Donkey anti-mouse IgG Secondary Antibody (clonality unspecified) | LI-COR | 926–32212 | (1:10,000) |
| recombinant DNA reagent | pBID-Ubi | this paper | modified from Addgene Plasmid #35,200 | |
| gene (D. melanogaster) |
RpL10Aa | NA | FBgn0038281 | |
| genetic reagent (D. melanogaster) |
attP40 | Model System Injections | y1() M{RFP[3xP3.PB] GFP[E.3xP3]=vas int.Dm}ZH2A w[*]; P{y[+t7.7]=CaryP}attP40 | |
| genetic reagent (D. melanogaster) |
w1118 | BDSC | #3605 | |
| genetic reagent (D. melanogaster) |
Ubi-GFP0D | Sawyer et al., 2020 | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP30D | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP50D | Sawyer et al., 2020 | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP60Dv1 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP60Dv2 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP60Dv3 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP70D | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP80D | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP90D | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP100D | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP50C3’ | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP60C3’ | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP70C3’ | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP80C3’ | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP90C3’ | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP50C5’ | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-GFP54C3’ | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv1 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv2 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv3 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv4 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv5 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv6 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv7 | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-mGFP100Dv8 | this paper | attP40 (2 L), attP2 (3 L) | |
| genetic reagent (D. melanogaster) |
Ubi-3xFLAG-RpL10Aa Endo | this paper | attP40 (2 L) | |
| genetic reagent (D. melanogaster) |
Ubi-3xFLAG-RpL10Aa Com | this paper | attP40 (2 L) |