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. 2022 May 11;2022:2030818. doi: 10.1155/2022/2030818

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Copyright © 2022 Hao Shen et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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IFN-γ inhibited osteoclast differentiation and bone resorption in a dose-dependent manner. (a) Relative expression of osteoclast marker genes after 3 days of osteoclast differentiation. (b and c) Representative images of TRAP staining (b) and F-actin cytoskeleton staining (c) and quantitative analysis of osteoclast number per well (Oc. N/well). (d) Representative images of resorption pit assay and quantitative analysis of bone resorption area (the percentage of the resorbed pit area per total area). C: control (with M-CSF only); R: RANKL (with M-CSF and RANKL) (3 replicates, ^∗P < 0.05).