Figure 7.

Anti-CD119 antibody counteracted the inhibitory effect of IFN-γ on osteoclast differentiation. BMMs were differentiated into osteoclasts with IFN-γ (10 ng/mL) and anti-CD119 antibody (5 ng/mL). (a) Relative expression of osteoclast marker genes after 3 days of differentiation. (b and c) Representative images of TRAP staining (b) and F-actin cytoskeleton staining (c) and quantitative analysis of osteoclast number per well (Oc. N/well). (d) The expression and quantitative analyses of MMP-9, CTSK, and NFATc1 in nuclear extracts of BMMs by western blot performed after 6 days. C: control (with M-CSF only); R: RANKL (with M-CSF and RANKL) (3 replicates, ^∗P < 0.05).