Figure 8.

IFN-γ inhibited osteoclastogenesis by suppressing RANKL-mediated activation of the NF-κB and MAPK pathways. BMMs were differentiated into osteoclasts with IFN-γ (10 ng/mL). (a) The expression of p65, Pi-p65, IκB, Pi-IκB, p38, Pi-p38, ERK, and Pi-ERK in nuclear extracts of BMMs by western blot performed after 6 days. (b) Representative images of p65 translocation in the differentiated BMMs by immunocytochemical staining performed after 30 minutes. (c and d) Representative images and quantitative analyses of c-Fos (c) and TRAP (d) in differentiated BMMs by immunocytochemical staining performed after 2 days. C: control (with M-CSF only); R: RANKL (with M-CSF and RANKL) (3 replicates, ^∗P < 0.05).