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. 2022 May 11;2022:9925919. doi: 10.1155/2022/9925919

Figure 2.

Figure 2

Apatinib induces ferroptosis of glioma cells. All the data refer to 72 h in the presence and in the absence of apatinib. (a) Flow cytometry representative histograms of DCFH-DA-stained U251 cells. (b) Fold change of the mean fluorescence intensity of DCFH-DA-stained U251 cells. (c) Flow cytometry representative histograms of DCFH-DA-stained U87 cells. (d) Fold change of the mean fluorescence intensity of DCFH-DA-stained U87 cells. (e, f) MDA levels in U251 and U87 cells. (g, h) Fe levels in U251 and U87 cells. (i, j) GSH levels in U251 and U87 cells. (k, l) Western blotting was used to determine the levels of GPX4 and SLC7A11 in U251 and U87 cells. (m, n) Western blotting was employed to detect the expression levels of Keap1, Nrf2, p-VEGFR2, and VEGFR2 in U251 and U87 cells. ∗P < 0.05; ∗∗P < 0.01.