Extended Data Fig. 6. Substrate specificity of host and viral enzymes related to Acb1 and Apyc1.
a, Homologs of T4 Acb1 were expressed, purified, and tested for cleavage of 3′3′-cGAMP (left) and cAAA (right) by TLC. Data are representative of 2 independent experiments. b, Summary of the distribution of bacterial Apyc1 homologs among bacterial orders. c, Homologs of SBSphiJ Apyc1 were expressed, purified, and tested for cleavage of cAMP and cCMP by HPLC. Data are representative of 3 independent experiments. d, Summary of HPLC analysis of cNMP degradation by SBSphiJ Apyc1 and closely related B. subtilis MBL phosphodiesterases. Data are presented as mean ± s.d. from n = 3 independent replicates.