Extended Data Fig. 10. Generation and validation of phage T4 Δacb1.
a, Sequencing reads of WT and Δacb1 phage T4. b, TLC analysis of cyclic nucleotide cleavage by WT or Δacb1 phage T4 lysate. Data are representative of 2 independent experiments. c, Representative plaque assays of E. coli carrying a plasmid encoding an active or catalytically inactive CBASS operon from Y. aleksiciae d, Summary of plaque assay results of WT or Δacb1 phage T4 infection of E. coli carrying catalytically inactive CBASS operons from Y. aleksiciae or E. coli. Data are presented as mean ± s.d. from n = 4 (Y. aleksiciae operon) or n = 3 technical replicates (E. coli operon) and are representative of at least 3 biologically independent experiments.