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. 2022 Apr 8;605(7910):522–526. doi: 10.1038/s41586-022-04716-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, T4 Acb1 was expressed as an N-terminal 6×His-MBP-SUMO2 fusion and purified by Ni-NTA and separated from His-MBP-SUMO2 by size exclusion chromatography. b, Coomassie-stained SDS-PAGE analysis of fully purified T4 Acb1. c, TLC analysis of 3′3′-cGAMP degradation by T4 Acb1 at the indicated pH. d, TLC analysis of 3′3′-cGAMP degradation by T4 Acb1 supplemented with the indicated metal or EDTA at the following concentrations: 50 mM EDTA; 5 mM MgCl₂; 1 mM MnCl₂; 5 mM CaCl₂; 1 μM NiCl₂; 1 μM CuCl₂; or 1 μM ZnSO₄. Data in all panels are representative of at least 3 independent experiments.