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. 2022 Apr 28;28(5):946–957. doi: 10.1038/s41591-022-01786-3

Extended Data Fig. 1. Variable chain gene sequences and HLA specificity of a PIK3CA public NeoAg-specific TCR panel.

Extended Data Fig. 1

(a) Table listing TRAV, TRAJ, TRBV and TRBJ gene segment composition, CDR3 sequences, and CDR3 lengths for a panel of PIK3CA public NeoAg TCRs retrieved using the SIFT-seq discovery platform. (b) Representative FACS plots and (c) summary bar graphs demonstrating the specificity of PIK3CA public NeoAg TCRs 1-4 for HLA-A03 supertype members HLA-A*03:01, HLA-A*03:02, and HLA-A*11:01. The number of shared amino acids and % homology of each supertype member to HLA-A*03:01 are shown. T cells transduced with individual TCR panel members were co-cultured with mono-allelic cell lines co-expressing the indicated HLA-A03 supertype member and either Mut or WT PIK3CA. Numbers within each FACS plot and bar graph indicate the frequency of TNFα producing T cells after pre-gating on live+mTCR+CD8+ cells. Bar graphs displayed as mean ± SEM using n = 3 biologic replicates per condition. Data shown is representative of 2 independent experiments.