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Validation of DEGs by qPCR. Expression profile of selected genes identified from RNA-seq validated by qPCR is shown. Primary HTM cells were treated with 100 nM DEX or 0.1% ETH for 16 h and 7 d. Gene expressions were normalized to GAPDH (16 h group) and ACTB (7 d group), and analyzed using the 2−ΔΔCT method. The data is represented as mean ± SEM. *P < 0.01; **P < 0.005; ***P < 0.001; ****P < 0.0001. Paired 2-tailed Student’s t test.
