Table 2.

Reproducibility assay Ct values from two independent dilutions of RNA from virus isolate.

	Extraction 1: Duplex				Extraction 2: Duplex
	E		RNase P		E		RNase P
DILUTION	Ct	Average	Ct	Average	Ct	Average	Ct	Average
1 × 10−1	12.4	12.6	28.3	28.4	12.6	12.6	28.3	28.3
	12.7		28.4		12.6		28.2
1 × 10−2	15.8	15.7	27.9	27.7	15.8	15.8	27.6	27.7
	15.7		27.5		15.8		27.9
1 × 10−3	19.2	19.2	27.1	27.2	19.1	19.1	27.4	27.4
	19.1		27.3		19.2		27.4
1 × 10−4	22.5	22.4	27.6	27.5	22.5	22.6	27.8	27.7
	22.3		27.5		22.6		27.6
1 × 10−5	26.0	25.8	28.2	28.1	25.8	25.7	28.2	28.1
	25.5		28.0		25.6		28.0
1 × 10−6	29.8	29.1	28.1	28.1	28.4	28.5	28.2	28.2
	28.3		28.1		28.6		28.1
1 × 10−7	35.6	32.9	28.1	28.1	33.6	32.4	28.1	28.1
	30.3		28.1		31.2		28.2
1 × 10−8	36.1	38.1	No data	28.2	0	0	28.2	28.2
	40.2		28.2		0		28.1
NTC	0	0	0	0	0	0	0	0