Table 3.
Comparison of Ct values obtained from monoplex and duplex PCR for clinical samples.
| Monoplex | Duplex |
Monoplex | ||
|---|---|---|---|---|
| Code | Sarbeco E | E | (RNase P) | RdRp |
| 1 | 21 | 23 | 22 | 25 |
| 2 | 0 | 0 | 29.8 | 0 |
| 3 | 30 | 30 | 30 | 32 |
| 4 | 0 | 0 | 30 | 0 |
| 5 | 0 | 0 | 31 | 0 |
| 6 | 0 | 0 | 31 | 0 |
| 7 | 0 | 0 | 33 | 0 |
| 8 | 0 | 0 | 26 | 0 |
| 9 | 0 | 0 | 23 | 0 |
| 10 | 0 | 0 | 31 | 0 |
| 11 | 23 | 23 | 0 | 24.8 |
| 12 | 26.7 | 26.4 | 27.6 | 28.5 |
| 13 | 23.7 | 23.8 | 33.2 | 25.1 |
| 14 | 19.5 | 19.4 | 26.8 | 21.5 |
| 15 | 0 | 0 | 29.1 | 0 |
| 16 | 0 | 0 | 31 | 0 |
| 17 | 0 | 0 | 32.8 | 0 |
| 18 | 0 | 0 | 30.5 | 0 |
| 19 | 0 | 0 | 22.6 | 0 |
| 20 | 0 | 0 | 27.5 | 0 |
| 21 | 0 | 0 | 26.6 | 0 |
| 22 | 0 | 0 | 25 | 0 |
| 23 | 0 | 0 | 27.8 | 0 |
| 24 | 0 | 0 | 25.5 | 0 |
| 25 | 0 | 0 | 26.7 | 0 |
| 26 | 18.2 | 18.7 | 27 | 25.6 |
| 27 | 25.8 | 25.4 | 26.7 | 29 |
| 28 | 26.5 | 26.8 | 31.3 | 31.4 |
| 29 | 20.2 | 20.7 | 31.9 | 23.4 |
| 30 | 23.8 | 23.5 | 33.9 | 26.5 |
| 31 | 27.8 | 28.1 | 32.5 | 31.2 |
| 32 | 21.8 | 21.8 | 0 | 24.9 |
| 33 | 17.7 | 17 | 33.1 | 17.6 |
| 34 | 20.5 | 20.8 | 26 | 24.3 |
| 35 | 20.2 | 20.2 | 0 | 24.3 |
| 36 | 25.6 | 26 | 32.5 | 29.8 |
| 37 | 17.2 | 17.3 | 24.6 | 17.6 |
| 38 | 25.2 | 31 | 23.6 | 30.4 |
| 39 | 0 | 0 | 25.6 | 0 |
| 40 | 0 | 0 | 26.5 | 0 |
| 41 | 0 | 0 | 27.1 | 0 |
| 42 | 21.1 | 21.3 | 24.3 | 22.3 |
| 43 | 26.2 | 28.7 | 25.7 | 28.8 |
| 44 | 26.2 | 27 | 26.7 | 28.5 |
| 45 | 26 | 25.9 | 27.8 | 25.8 |
| 46 | 27.6 | 27.9 | 28.4 | 26.8 |
| 47 | 31.5 | 35 | 29.9 | 0 |
| 48 | 0 | 36 | 29.9 | 0 |
| 49 | 0 | 36.2 | 29.4 | 0 |
| 50 | 24.2 | 24.7 | 27 | 24.8 |
| 51 | 21 | 21.6 | 26.4 | 22.2 |
| 52 | 0 | 0 | 27.4 | 0 |
| 53 | 0 | 36.2 | 25.7 | 0 |
| 54 | 33 | 32 | 25.8 | 0 |
| 55 | 0 | 0 | 28.3 | 0 |
| 56 | 31.1 | 30.6 | 28.6 | 34.4 |
| 57 | 32 | 32.6 | 31.3 | 34.4 |
| 58 | 31 | 32.4 | 25.2 | 33.2 |
| 59 | 31.9 | 33.9 | 27.3 | 34.7 |
| 60 | 31.4 | 36.3 | 26 | 33.8 |
| 61 | 32.3 | 33.7 | 31.6 | 33.9 |
| 62 | 30 | 32.4 | 26.3 | 32.7 |
| 63 | 33.2 | 33.6 | 27.1 | 34 |
| 64 | 36.7 | 27.9 | 28.4 | 0 |
| 65 | 0 | 35.2 | 29.9 | 0 |
| 66 | 34.1 | 0 | 25.9 | 0 |
| 67 | 37.8 | 36.2 | 30.6 | 0 |
| 68 | 0 | 34.1 | 31.7 | 0 |
| 69 | 0 | 0 | 30.6 | 0 |
| 70 | 0 | 0 | 32.6 | 0 |
| 71 | 20.8 | 21 | 32.2 | 26.8 |
| 72 | 34.7 | 0 | 36 | 0 |
| 73 | 14.2 | 14.4 | 29.1 | 20.5 |
| 74 | 0 | 0 | 26.5 | 0 |
| 75 | 36.3 | 0 | 28.3 | 0 |
| 76 | 0 | 0 | 30.6 | 0 |
| 77 | 0 | 0 | 32.3 | 0 |
| 78 | 37.6 | 0 | 30.7 | 0 |
| 79 | 0 | 0 | 30.2 | 0 |
| 80 | 35.4 | 35 | 30.4 | 0 |
| 81 | 37.1 | 0 | 32 | 0 |
| 82 | 37.3 | 37.8 | 25.3 | 0 |
| 83 | 0 | 0 | 30.1 | 0 |
| 84 | 0 | 0 | 29.8 | 0 |
| 85 | 0 | 0 | 30.6 | 0 |
| 86 | 32.4 | 32.3 | 32.6 | 37.5 |
| 87 | 23.5 | 23.5 | 31.2 | 29.1 |
| 88 | 22.3 | 22.3 | 29.1 | 28.2 |