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. 2022 May 18;13:2725. doi: 10.1038/s41467-022-30008-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Illustration of the 12 clones that were used to generate the A375 Signalome cell line. A375 cells were first infected with iRFP-H2A to mark the cell nucleus. Then, 12 clones were generated with 12 visual barcodes. Lastly, a different live reporter was added to each of the clones. Transcription activating reporters are represented by gold while translocation reporters are represented in red. Binding partners in the nucleus are represented in purple. B Relative number of cells from each clone in a DMSO control wells over time. C Scatter plot showing the correlation between the reporter activity scores, for all 12 clones when grown separately or as part of the Signalome cell line, in response to 75 drugs. D–F Reporter activity plots of the A375 signalome cell line in response to DMSO, vemurafenib (1 µM) or trametinib (0.125 µM) over time. Blue and red backgrounds represent activation or inhibition scores above 0.2 or below −0.2, respectively. The average cell count per reporter in (D–F) is: 656, 545, and 530 cells, respectively. Source data are provided with this paper.