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Phage isolation using double layer agar assay and their transmission electron micrograph (TEM). A, C, E Three double layered agar plates showing different types of phage plaque morphologies isolated directly from river water. B TEM of Escherichia phage vB_EcoM_TU01 (scale bar = 100 nm), D TEM of Klebsiella phage vB_KpnM_TU02 (scale bar = 100 nm), F TEM of Salmonella phage vB_SalS_TU03 (scale bar = 20 nm)
