TABLE 2.

Microsatellite sequences and the primers used to amplify these loci

Locusa	Microsatellite sequence	Type	Primer Sequence	Tmb	Product length (bp)	Isolate
B1c	(AAG)18	B1.1	5′-CGC ACA ATA CGT CAG CTA GGA ATG	55	325	NF 62A
		B1.2	5′-CCT GAA TCA ACT TTG CTA TCA GGC	54
B2	(AC)18	B2.1	5′-TCC GTA ATG CTC TGC TTT GGC AGG	57	163	E8
		B2.2	5′-CTG CTG ACA GTA GGT GGA TGA TGG	57
B3	C12 and (GA)7	B3.1	5′-TGC TGT CCG TTC TCG TGG CAT TCG	59	115	E8
		B3.2	5′-CGG TGT AGA AGG ACC TGC AGT TTG	57
B4	(CA)3GCG(CA)3ACG(CA)3A(CA)4	B4.1	5′-TGG ACT CGA CTT GCC CTC TCT CAG	59	124	E8
		B4.2	5′-TGC GAG CAG CGT TTG CGT GTG CGT	61
B5	(CATCTCATCA)5	B5.1	5′-CAA CTC CAA CAA ACT CAA CCA GCG	55	107	E8
		B5.2	5′-GGA CTC GTG CAA AGC TTC GGA TGG	59
B6	(CAT)2CAC(CAT)3	B6.1	5′-GGC ATG GTA TGG GCA ATG AGT GTC	57	173	E8
		B6.2	5′-CTG CTG CGA TGT TTT GTA CTG TGG	55
B7	(AG)5A(AG)3	B7.1	5′-AAC TTG AAT TAA CTA GGG GCT AGG	52	111	Lp1
		B7.2	5′-TTA ATT CGC TAC CCT CTC TTT TGC	52
B8	(AC)8T6	B8.1	5′-CTA ACA CGC TCA AGG GAG ATT GCC	57	142	Lp1
		B8.2	5′-GGC GAG GTG CAA CCC TGT TAA TGG	59
B9	G5AG9 and (GAGAG)2C(GAGGA)2	B9.1	5′-AAT CGT TGT GCG AGC CAT TCT GGC	57	167d	Lp1
		B9.2	5′-TCC ATC TCC GCA ATC TGC ATG TCC	57
		B9.4	5′-GCC CCG TCA TGC ATT ATC TCC TTG	57	246e	Lp1
B10	(AGC)3CG(CAT)3(CAA)5	B10.1	5′-CGC TCA GGG CTA CAT ACA CCA TGG	59	179	Lp1
		B10.2	5′-CTC ATC GAG TAA CGC AGG CGA CG	59
B11	(GACA)18	B11.1	5′-CAT GGA TGG ACA AGA GAT TGC ACG	55	173	Lp19
		B11.4	5′-TTC ACT GCT ACA ATT CTG TCC AGC	54

^aB2, B3, B4, B7, B8, and B9 were isolated by using the dinucleotide probe mixture of (CA)₁₅ and (GA)₁₅. B5, B6, and B10 were isolated by using the trinucleotide probe mixture of (CAA)₁₀, (GAA)₁₀, and (ATC)₁₀. 

^bT_m is calculated by using as follows: T_m = 81.5 + 16.6(log₁₀[Na⁺]) + 0.41(%G + C) − 600/N (5), where [Na⁺] is 0.05 M and N is the length of the oligonucleotide. 

^cLocus isolated by Groppe et al. (27) from Epichloë mating population VI (52). 

^dExpected product size for B9.1-B9.2 primer pair. 

^eExpected product size for B9.1-B9.4 primer pair.