Efficacy of MPs co-loaded with phage active against S. aureus and P. aeruginosa against: A, B, mixture of PAO1-GFP and UAMS-1; and C, D, UAMS-1 and PA103. 50 μL of UAMS-1 (1×107 CFU/mL) in LB and 50 μL of PAO1-GFP (7×106 CFU/mL) or PA103 (1×107 CFU/mL) was treated with MP or phage-MP (2 mg/mL). For A and C, Normalized optical density was measured at 630 nm over a period of 15 hours. For B and D, the suspensions were serially diluted, plated on agar plates, and incubated at 37 °C, followed by enumeration of bacterial colonies after 16–20 hours. Dashed line represents limit of detection. Two-way ANOVA with Sidak’s test for multiple comparison. Two-way ANOVA with Dunnett’s multiple comparison test relative to time 0. ****P < 0.0001, ***P < 0.001 via unpaired two-tailed t test, N = 3–6.