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. Author manuscript; available in PMC: 2022 Oct 25.
Published in final edited form as: Nat Genet. 2022 Apr 25;54(5):670–683. doi: 10.1038/s41588-022-01045-8

Extended Data Fig. 5. HOXB13 KD induces de novo lipogenesis in PCa cells cultured in either lipid-free or regular medium.

Extended Data Fig. 5

a-e. LipidSpot staining and quantification of lipid droplets in control (sgNC) or HOXB13 KD (sgHOXB13) LNCaP (a-c) and PC-3M (d-f) cultured in lipid-free or regular medium (10% FBS). WB (a,d) validating FASN up-regulation in HOXB13-KD cells cultured in lipid-free medium, confirming de novo lipogenesis. Data (b,e) shown are representative images of LipidSpot staining with a scale bar of 20 μm. LipidSpot staining intensity was quantified and calculated as fold change of LipidSpot staining in each condition normalized to sgNC non-target control under lipid-free medium (c,f). Each data point represents an average of 6 (n=6) fields per independent experiment by image J. Data are the mean ±s.d. Unpaired two-sided t-test were performed between indicated groups as show in figure. g. Quantification of lipid accumulation of PDXs in Fig.4i. Six (n=6) representative areas per PDX were quantified using Image J. Data are shown as mean±s.d. Unpaired one-sided t-test was performed between indicated groups as show in figure.