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Maturation of HydA without lysate, using the GCS H-protein. Without H-protein (Ctrl) or with lipoyl-H-protein (Hred) no active HydA is formed. Addition of aminomethyl-lipoyl-H-protein (Hmet) provides maturation of HydA in a concentration-dependent manner. Assays for H2 were run for 24 h to allow H2 to accumulate. Conditions as reported in the SI.
