Table 1.
Body Characteristics and Echocardiography Measurements for Non-Tg, M3-SIRT3 and M1-SIRT3 Mice Treated with 8.0mg/kg of DOX for 4 Weeks.
| Genotype-Treatment | Non-Tg-CON | Non-Tg-DOX | M3-SIRT3-CON | M3-SIRT3-DOX | M1-SIRT3-CON | M1-SIRT3-DOX |
|---|---|---|---|---|---|---|
| Number of animals | (n=8) | (n=8) | (n=8) | (n=9) | (n=9) | (n=10) |
| Body Characteristics | ||||||
| Body weight (g) | 24.8±2.9 | 17.0±2.4* | 24.2±2.3† | 19.2±2.5*,‡ | 24.1±3.5† | 19.8±2.2*,‡ |
| Heart weight (mg) | 123±17 | 94±12* | 125±25† | 97±14*,‡ | 134±13† | 114±10 |
| HW/BW (%) | 0.50±0.08 | 0.56±0.09 | 0.52±0.10 | 0.51±0.09 | 0.57±0.11 | 0.57±0.06 |
| Cardiac Structure | ||||||
| LVPWd (mm) | 0.71±0.08 | 0.59±0.07* | 0.69±0.08 | 0.64±0.08 | 0.70±0.07† | 0.67±0.12 |
| LVAWs (mm) | 1.06±0.09 | 0.88±0.15* | 1.05±0.14 | 0.97±0.19 | 1.13±0.13† | 1.10±0.15† |
| LVAWd (mm) | 0.74±0.03 | 0.61±0.05 | 0.74±0.09 | 0.66±0.12 | 0.81±0.15† | 0.73±0.11 |
| LVIDs (mm) | 2.79±0.30 | 2.80±0.24 | 2.65±0.34 | 2.67±0.33 | 2.98±0.19 | 2.84±0.14 |
| LVIDd (mm) | 3.85±0.28 | 3.74±0.33 | 3.81±0.28 | 3.75±0.24 | 3.95±0.18 | 3.92±0.23 |
| IVSs (mm) | 0.95±0.09 | 0.80±0.10 | 0.98±0.05 | 0.96±0.11 | 0.99±0.20† | 1.02±0.14† |
| IVSd (mm) | 0.66±0.07 | 0.58±0.01 | 0.66±0.05 | 0.64±0.12 | 0.73±0.17 | 0.72±0.11 |
| LV Vols (μL) | 29.8±8.1 | 29.8±6.0 | 26.4±8.1 | 26.8±7.7 | 34.7±5.4 | 30.8±3.8 |
| LV Vold (μL) | 64.3±11.7 | 60.3±12.1 | 62.8±10.4 | 60.3±8.9 | 68.2±7.4 | 67.1±9.5 |
| Cardiac Function | ||||||
| CO (mL/min) | 18.6±2.4 | 12.8±3.6* | 18.4±2.7† | 16.8±2.7 | 17.9±2.6† | 19.7±4.2† |
| FS (%) | 29.1±2.5 | 23.9±2.0* | 30.3±4.0† | 28.9±4.1† | 26.2±3.0 | 28.1±3.3 |
| SV (μL) | 36.9±4.8 | 28.5±5.8 | 36.6±4.8 | 34.3±4.8 | 36.4±5.6 | 38.2±8.6† |
| E’/A’ Ratio | 1.4±0.3 | 1.1±0.2* | 1.5±0.3† | 1.4±0.3 | 1.4±0.3 | 1.4±0.2 |
| LV MPI | 0.45±0.08 | 0.65±0.10* | 0.43±0.12† | 0.41±0.06† | 0.41±0.10† | 0.42±0.08† |
| BPM | 505±20 | 445±52* | 501±32 | 489±39 | 492±28 | 518±49† |
HW, Heart Weight; BW, Body Weight; LVAW, left ventricular anterior wall; LVPW, left ventricular posterior wall; IVS, intraventricular septum; LV Vol, left ventricular volume; s, systole; d, diastole; CO, cardiac output; FS, fraction shortening; SV, stroke volume; LVMPI, left ventricular myocardial performance index; BPM, beats per minute. Female mice.
*
p<0.05 vs Non-Tg-CON,
†
p<0.05 vs Non-Tg-DOX,
‡
p<0.05 vs related CON group, values are mean +/− SD. Comparisons by Two-way ANOVA with Tukey’s post-hoc test.