Table 2.

Differentially acetylated peptides identified with LC-MS/MS after DOX treatment in Non-Tg Mice

Description	Gene ID	Protein ID*	Peptide Sequence†	Lys-Acetyl Location‡	Fold Change§
Hyperacetylated
Isocitrate dehydrogenase [NADP]	Idh2	P54071	TDFDkNK	K280	6.26
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit	Sdhb	Q9CQA3	kFAIYR	K43	2.62
Citrate synthase	Cs	Q9CZU6	TFkQQHGK	K53	2.22
Dihydrolipoyl dehydrogenase	Dld	O08749	MMEQkHSAVK	K127	2.00
Trifunctional enzyme subunit alpha,	Hadha	Q8BMS1	VLkEVESVTPEHCIFASNTSALPINQIAAVSK	K460	1.64
Electron transfer flavoprotein-ubiquinone oxidoreductase	Etfdh	Q921G7	FAILTEkHR	K152	1.56
Aspartate aminotransferase	Got2	P05202	kAEAQIAAkNLDK	K82, K90	1.49
Aconitate hydratase	Aco2	Q99KI0	AIITkSFAR	K689	1.33
Short-chain specific acyl-CoA dehydrogenase	Acadsd	Q07417	AAMLkDNK	K335	0.95
Malate dehydrogenase, cytoplasmic	Mdh1	P14152	SQGTALEkYAK	K118	0.69
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit	Sdhb	Q9CQA3	WDPDkTGDKPR	K53	0.58
Trifunctional enzyme subunit alpha	Hadha	Q8BMS1	TSkDTTASAVAVGLR	K519	0.48
Hypoacetylated
Trifunctional enzyme subunit alpha	Hadha	Q8BMS1	ILQEGVDPkK	K569	−0.28
ATP synthase subunit epsilon	Atp5e	P56382	FSQICAkAVR	K21	−0.68
Isocitrate dehydrogenase [NADP]	Idh2	P54071	GkLDGNQDLIR	K384	−0.77
Cytochrome c, somatic	Cycs	P62897	MIFAGIkK	K87	−0.83
Cytochrome c oxidase subunit 4 isoform 1	Cox4i1	P19783	EKEkADWSSLSR	K67	−0.87
Superoxide dismutase [Mn]	Sod2	P09671	GELLEAIkR	K122	−0.88
Trifunctional enzyme subunit alpha	Hadha	Q8BMS1	KYESAYGTQFTPCQLLLDHANNSSKk	K760	−0.91
Electron transfer flavoprotein subunit alpha	Etfa	Q99LC5	LGGEVSCLVAGTkCDK	K59	−1.12
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2	Ndufa2	Q9CQ75	ECSEVQPkLWAR	K64	−1.15
Trifunctional enzyme subunit alpha	Hadha	Q8BMS1	FVDLYGAQkVVDR	K728	−1.23
Fumarate hydratase	Fh	P97807	KPVHPNDHVNkSQSSNDTFPTAMHIAAAVEVHK	K180	−1.24
NADH-ubiquinone oxidoreductase 75 kDa subunit	Ndufs1	Q91VD9	MCLVEIEkAPK	K84	−1.29
Protein NipSnap homolog 2	Gbas	O55126	LQFHNVKPECLDAYNkICQEVLPK	K86	−1.42
Aspartate aminotransferase	Got2	P05202	ASAELALGENNEVLkSGR	K122	−1.45
CDGSH iron-sulfur domain-containing protein 1	Cisd1	Q91WS0	VVHAFDMEDLGDkAVYCR	K68	−1.47
Isocitrate dehydrogenase [NADP	Idh2	P54071	NTILkAYDGR	K256	−1.50
ATP synthase subunit alpha	Atp5a1	Q03265	DNGkHALIIYDDLSK	K315	−1.63
Aspartate aminotransferase	Got2	P05202	LTkEFSVYMTK	K396	−1.74
Delta-1-pyrroline-5-carboxylate dehydrogenase	Aldh4a1	Q8CHT0	ETHkPLGDWR	K560	−1.77
Malate dehydrogenase	Mdh2	P08249	VNVPVIGGHAGkTIIPLISQCTPK	K213	−1.78
Isocitrate dehydrogenase [NADP]	Idh2	P54071	VEkPVVEMDGDEMTR	K48	−1.97
Isocitrate dehydrogenase [NADP]	Idh2	P54071	FAQTLEkVCVQTVESGAMTK	K400	−2.05
Malate dehydrogenase	Mdh2	P08249	ANVkGYLGPEQLPDCLK	K78	−2.08
Malate dehydrogenase	Mdh2	P08249	ANTFVAELkGLDPAR	K185	−2.43

^*Protein ID refers to UniProt ID.

^†Peptide sequence: Bolded lowercase k represents position of acetylation in peptide sequence. Location refers to amino acid position within total protein peptide sequence.

^‡Lys-Acetyl: Lysine Acetylation. Location identified using UniProt.

^§Fold change calculated DOX-CON using normalized and scaled down log2 values. Statistics are unpaired student t-test. P<0.05, Non-Tg-CON n=6 (males), Non-Tg-DOX n=5 (males).