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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Alcohol. 2022 Feb 26;101:17–26. doi: 10.1016/j.alcohol.2022.02.003

Figure 3. Ethanol CPP decreases PV-IN current-evoked spiking.

Figure 3.

(a) Top, Representative PV-IN current-clamp recordings from control (left, grey/black) and CPP mice (right, red). Traces depict responses to current injections. The responses to 0 pA and 100 pA injections are shown on top and the responses to 200 pA are shown on the bottom. Scale bars 20 mV, 0.5 s. (b) CPP decreased spike-firing in response to current injections (RM Three-way ANOVA: F14,616 = 2.2, **:p<0.01 CPP x current interaction). PV-INs from female mice were more excitable than those from male mice. (RM Three-way ANOVA: F14,616 = 2.3, **:p<0.01 sex x current interaction). (c) PV-INs from female mice were more excitable than those from male mice (RM Three-way ANOVA: F14,616 = 5.7, ****:p<0.0001 sex x current interaction), but no effect of CPA was detected (RM Three-way ANOVA: F14,616 = 0.5, n.s. CPA x current interaction). The same control data are presented in panels B and C. (d) PV-INs from female mice were more depolarized than those from male mice (Two-way ANOVA: F1,66 = 5.2, *:p<0.05 main effect of sex), but no effect of ethanol conditioning was detected. (e) PV-INs from female mice displayed lower threshold currents to fire (Two-way ANOVA: F1,66 = 14.5, ***:p<0.001 main effect of sex), but no effect of ethanol conditioning was detected. n/N = 9-15 cells from 3-5 mice per group.