Skip to main content
. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Gastroenterology. 2022 Feb 12;162(7):1962–1974.e6. doi: 10.1053/j.gastro.2022.02.016

Figure 2. Decreased colonic SERT expression induced by IBS-D fecal supernatant is mast cell–dependent.

Figure 2.

(A) RT–PCR of SERT in murine colonic organoids alone or co-cultured with mast cells (MC), treated with fecal supernatant (FS) from HC or IBS-D patients. Results are expressed as mean ± SEM, two-way ANOVA, n = 6 in each group. (B) colonic SERT expression in response to intracolonic infusion of IBS-D FS in wild-type (WT), W/Wv, W/Wv mice reconstituted with bone marrow–derived MC from wild-type mice, and W/Wv mice reconstituted with bone marrow–derived MC from TLR deficient mice. Results are expressed as mean ± SEM, one-way ANOVA, n = 4–6 in each group. (C) Decreased colonic SERT expression evoked by intracolonic IBS-D FS infusion was abolished by ketotifen or cromolyn sodium pretreatment (30 min before infusion, IP). Results are expressed as mean ± SEM, one-way ANOVA, n = 4–7 in each group.