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. 2022 Apr 18;298(6):101950. doi: 10.1016/j.jbc.2022.101950

Figure 1.

Figure 1

SCLC and NSCLC cells secrete qualitatively different sEVs.A, schematic representations of oligomannose-type and complex-type N-glycans. Sialic acids (Sia) are linked to galactose (Gal) via α2,3 or α2,6 linkage. β1,2- and β1,4-linked GlcNAc branching occurs on the α1,3-mannose (Man) arm of N-glycan. β1,2- and β1,6-linked GlcNAc branching occurs on the α1,6-Man arm of N-glycan. α1,6-core fucosylation occurs on the innermost GlcNAc residue. B, Western blot analysis of sEVs (5 μg of protein for left panels or 15 μg (3×) of protein for right panels) released from SCC (H520, SK-MES-1, and LK-2), LCC (H1299), LUAD (HCC827), and SCLC (H446 and SBC-3) cells. n.d., not detected. C, transmission electron microscopy (TEM) analysis of sEVs prepared in (B). The scale bars represent 200 nm. Western blots are representative images from three independent experiments. TEM analysis was performed once. LCC, large-cell lung carcinoma; LUAD, lung adenocarcinoma; NSCLC, non–small-cell lung carcinoma; SCC, squamous-cell lung carcinoma; SCLC, small-cell lung carcinoma; sEV, small extracellular vesicle.