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. 2022 Apr 18;298(6):101950. doi: 10.1016/j.jbc.2022.101950

Figure 3.

Figure 3

SCLC-sEVs and NSCLC-sEVs express several integrin subunits.A and B, lectin blot analysis of whole-cell lysates (C, 20 μg) and sEVs (E, 5 μg) using SSA (A) and WGA (B) lectins. C and D, lectin pull-down (LP) of glycoproteins from the detergent-solubilized sEVs using SSA-conjugated beads (for H520-sEVs) or WGA-conjugated beads (for H446-sEVs), followed by SDS-PAGE/silver staining. Arrows indicate integrin subunits detected in the corresponding bands in proteomics. E and F, validation of integrin expression by Western blot analysis. Whole-cell lysates (C, 20 μg) and sEVs (E, 5 μg) from H520 (E) and H446 (F) cells were analyzed. See also Data S2 and S3. Lectin blots (A and B) are representative images from three independent experiments. Experiments for (C−F) were performed once. NSCLC, non–small-cell lung carcinoma; SCLC, small-cell lung carcinoma; sEV, small extracellular vesicle; SSA, Sambucus sieboldiana; WGA, wheat germ agglutinin.