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. 2022 May 5;13:850022. doi: 10.3389/fphys.2022.850022

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Copyright © 2022 Yao, Lin, Cai, Wang, Hou, Hu, Gao and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of S. frugiperda dsRNases at different ages. Using egg, first instar larvae (first), second instar larvae (second), third instar larvae (third), fourth instar larvae (fourth), fifth instar larvae (fifth), and sixth instar larvae (sixth) as templates for qPCR and using the eggs as a control to calculate the dsRNases in different instars. The relative expression level of mRNA (A–D). Five larvae are a biological replicate, and this experiment was performed three times in total. The data shown are mean ± SE, n = 21, different letters indicate a significant difference among ages [p < 0.05, one-way ANOVA followed by Duncan’s multiple range test for (A,B,C)].