FIGURE 7.

In vitro incubation of dsRNA and midgut juice and hemolymph. Relative content of dsEGFP in the control group = 1. The relative content of dsRNA was negatively correlated with the ability to degrade dsRNA. In vitro incubation of dsRNA and midgut fluid, dsRNases are the midgut extract of S. frugiperda larvae after successfully interfering with 4 sfdsRNase genes. At the same time, to avoid the influence of dsEGFP injection on the detection of dsEGFP content after incubation, the larval midgut fluid injected with water was used as a control. The midgut extract was incubated with dsEGFP for 10 and 60 min at room temperature, and the midgut juice was replaced by water as a control. The dsRNA content after incubation was detected by qPCR, and the relative content ratio was calculated [(A) represented relative content of dsEGFP after 10 min in midgut, (B) represented relative content of dsEGFP after 60 min in midgut, (C) represented relative content of dsEGFP after 10 min in hemolymph, (D) represented relative content of dsEGFP after 60 min in hemolymph]. The data shown are mean ± SE, n = 12 for the dsRNA relative content ratio calculation, different letters indicate a significant difference among treatments [p < 0.05, one-way ANOVA followed by Duncan’s multiple range test for (A,B)].