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. 2022 May 5;12:862564. doi: 10.3389/fonc.2022.862564

Figure 6.

Figure 6

MiR-195-5p modulates the proliferation and migration of LUAD by Targeting PTBP1. (A) Identity the PTBP1 as the target gene of miRNA-195-5p by employed diverse public databases. (B) The correlation between the miRNA-195-5p and PTBP1 in TCGA LAUD data. (C) The target sites between the miRNA-195-5p and PTBP1 were predicted by starBase. (D) The expression of PTBP1 in LUAD cell after over-expression of miRNA-195-5p was examined by the qRT-PCR assay. (E)The luciferase activities of the PTBP1 luciferase reporter vector (WT or MUT) in A549 cells transfected with miRNA-195-5p mimics or mimics NC. (F) The over-expression efficient of PTBP1 in A549 cells examine by qRT-PCR assay. (G) Overexpression of PTBP1 could reverse the repressed cell proliferation induced by miR-195-5p overexpression in A549 cells examined by growth curve assay. (H) Overexpression of PTBP1 could reverse the repressed cell migration induced by miR-195-5p overexpression in A549 cells examined by transwell assay. (I) The expression of PTBP1 in A549 cells after transfection miR-195-5p mimics and over-expression of PTBP1. Quantification data were also indicated. (J) Overexpression of PTBP1 could reverse the repressed cell migration induced by miR-195-5p overexpression in A549 cells examined by wound healing assay. Scale bar=50 μm. ***P < 0.001; NS, p > 0.05.