Fig. 3.

(A) The conservation score of each position in a multiple sequence alignment for AT₂R sequences. Secondary structures for positions were labelled above. Color scheme: green, α-helix; purple, β-sheet; gray, loop. (B) Logoplots visualizing residue frequency in each position. (C) SCA matrix for residue pairs. From blue to red, the evolutional correlation increases. H8 interaction domain is labelled by a line. (D) The projection of residues on the PC2-PC3 and PC2-PC4 decomposition for SCA matrix. Blue and red circles identify corresponding sector residues. (E) The clustered co-evolution matrix for blue and red sectors. (F) The location of blue and red evolutional sectors on AT₂R. Sector residues are colored correspondingly and the other residues are shown in gray cartoons. Key helixes and position of the orthosteric pocket are labelled. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)